Nthy-ori 3-1-CAS9 cell line

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Nthy-ori 3-1-CAS9 Order

Catalog#

YC-C089-Cas9-H

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

Cas9 Stable Cell Lines

cell name

Nthy-ori 3-1-CAS9

Cell Morphology

Epithelial-like,adherent

Passage ratio

1:2~1:4

Species

Human

Expression gene

Cas9

Resistance gene

hygro

Construction method

Lentivirus

Mycoplasma test

negative

Culture method

90%RPMI-1640+10% FBS

Description

Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the Nthy-ori 3-1 cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a Nthy-ori 3-1-CAS9 cell line that can maintain long-term, stable and high expression.

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:Nthy-ori 3-1			STR Info (Cell bank) Cell Line:Nthy-ori 3-1
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	11		11	11
D13S317	11	11		11	11
D7S820	7	12		7	12
D16S539	12	13		12	13
VWA	16	18		16	18
TH01	7	7		7	7
AMEL	X	X		X	X
TPOX	9	9		9	9
CSF1PO	12	12		12	12
D12S391	17.3	20
FGA	21	22
D2S1338	20	26
D21S11	29	30
D18S51	14	14
D8S1179	12	12
D3S1358	14	16
D6S1043	11	11
PENTAE	7	13
D19S433	16.2	16.2
PENTAD	8	13
D1S1656	15.3	17.3

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
01
High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
02
High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
03

Nthy-ori 3-1-CAS9

Catalog#

YC-C089-Cas9-H

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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Nthy-ori 3-1-CAS9 cell line

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